高階多維度影像分析平台
High-Dimensional Advanced Imaging Core Facility
I11-Nikon倒立電動顯微鏡系統
Nikon Ti2-E Motorized Inverted Microscope
Nikon Ti2-E
儀器描述
Nikon Eclipse Ti2-E 為倒立式螢光顯微鏡,本系統搭配高靈敏背照式單色PCO Edge 4.2 Bi影像系統,全電動XYZ多通道螢光轉盤系統,可設定不同位置多點多螢光通道拍攝,亦可進行大範圍拼圖及縮時攝影。
主要特點
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可進行組織樣品大面積快速拼圖(Image Tiling)
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sCMOS弱螢光影像擷取
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LED螢光光源多通道拍攝(Multi-Channel)
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可進行長時間活細胞實驗
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多種物鏡可供選擇(空氣鏡/水鏡/油鏡)
儀器規格
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電動倒立型顯微鏡:Inverted Motorized Microscope ECLIPSE Ti2-E
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電動Z軸及電動XY載物台
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電動PFS及時焦距回復系統
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DIC 微分干涉相位差模組(10X及40X)
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PH 相位差模組(10X及20X)
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物鏡: 10x (DIC), 20x (Ph2), 40x (DIC), 60x油鏡及 100x油鏡
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螢光濾片: DAPI, FITC, TRITC, Cy5
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LED多波段螢光光源,可切換395/25, 440/20, 470/24, 510/25, 550/15, 575/25, 640/30 nm
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影像擷取系統pco. edge 4.2 bi背照式高靈敏單色sCMOS相機
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影像擷取及分析軟體:Nikon NIS-Elements AR
注意事項
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封片樣品建議使用No. 1.5 (0.16-0.19mm)或No. 1.5H (0.17 mm) 蓋玻片。
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上機前確認mounting medium已固化或蓋玻片已固定確保mounting medium不會滲漏。
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水鏡/油鏡使用後請確實擦拭乾淨。
放置地點
國家生技研究園區A棟4樓A419室
管理人員
羅麗紋 博士
電話:02-7750-5554 或 02-7750-5556
Description
The Nikon Eclipse Ti2-E is an inverted fluorescence microscope system equipped with the high-sensitivity back-illuminated monochrome PCO Edge 4.2 Bi imaging system. This fully motorized XYZ microscope features a multi-channel fluorescence turret , enabling multi-point, multi-fluorescence channel imaging, large-area tiling, and time-lapse photography.
Key Features
- Rapid large-area image tiling for tissue samples
- sCMOS camera for capturing low fluorescence signals
- Multi-channel LED fluorescence light source
- Suitable for long-term live-cell experiments
- Various objective lenses available (air, water, and oil immersion)
Specifications
- Inverted Motorized Microscope: ECLIPSE Ti2-E
- Motorized Z-axis and XY stage
- Motorized PFS (Perfect Focus System) for real-time focus maintenance
- DIC (Differential Interference Contrast) module: 10X and 40X
- PH (Phase Contrast) module: 10X and 20X
- Objectives:
- 10X (DIC)
- 20X (Ph2)
- 20X water immersion
- 40X (DIC)
- 60X oil immersion
- 100X oil immersion
- Fluorescence Filters: DAPI, FITC, Cy5
- LED multi-band fluorescence light source: switchable between 395/25, 440/20, 470/24, 510/25, 550/15, 575/25, 640/30 nm
- Imaging System: pco.edge 4.2 bi high-sensitivity back-illuminated monochrome sCMOS camera
- Image Acquisition and Analysis Software: Nikon NIS-Elements AR
Precautions
- For slide-mounted samples, use No. 1.5 (0.16-0.19mm) or No. 1.5H (0.17mm) coverslips.
- Ensure the mounting medium is cured or the coverslip is secured to prevent leakage before imaging.
- Clean water and oil immersion lenses thoroughly after use.
Assistance Required
Please notify the administrator for assistance with the following:
- Non-standard slide-type samples
- Use of the live cell incubation system
Location
National Biotechnology Research Park, Building A, 4th Floor, Room A419
Contact Information
Dr. Li-Wen Luo
Email: llw@gate.sinica.edu.tw
Phone: +886-2-7750-5554 or +886-2-7750-5556