高階多維度影像分析平台

High-Dimensional Advanced Imaging Core Facility

I11-Nikon倒立電動顯微鏡系統

Nikon Ti2-E Motorized Inverted Microscope

Nikon Ti2-E

Nikon倒立電動顯微鏡系統

儀器描述

Nikon Eclipse Ti2-E 為倒立式螢光顯微鏡,本系統搭配高靈敏背照式單色PCO Edge 4.2 Bi影像系統,全電動XYZ多通道螢光轉盤系統,可設定不同位置多點多螢光通道拍攝,亦可進行大範圍拼圖及縮時攝影。

主要特點

  • 可進行組織樣品大面積快速拼圖(Image Tiling)

  • sCMOS弱螢光影像擷取

  • LED螢光光源多通道拍攝(Multi-Channel) 

  • 可進行長時間活細胞實驗

  • 多種物鏡可供選擇(空氣鏡/水鏡/油鏡)

儀器規格

  • 電動倒立型顯微鏡:Inverted Motorized Microscope ECLIPSE Ti2-E

  • 電動Z軸及電動XY載物台

  • 電動PFS及時焦距回復系統

  • DIC 微分干涉相位差模組(10X及40X)

  • PH 相位差模組(10X及20X)

  • 物鏡: ​​​​10x (DIC),  20x (Ph2), 40x (DIC), 60x油鏡及 100x油鏡

  • 螢光濾片: DAPI, FITC, TRITC, Cy5

  • LED多波段螢光光源,可切換395/25, 440/20, 470/24, 510/25, 550/15, 575/25, 640/30 nm

  • 影像擷取系統pco. edge 4.2 bi背照式高靈敏單色sCMOS相機

  • 影像擷取及分析軟體:Nikon NIS-Elements AR

注意事項

  • 封片樣品建議使用No. 1.5 (0.16-0.19mm)或No. 1.5H (0.17 mm) 蓋玻片。

  • 上機前確認mounting medium已固化或蓋玻片已固定確保mounting medium不會滲漏。

  • 水鏡/油鏡使用後請確實擦拭乾淨。

 

放置地點

國家生技研究園區A棟4樓A419室

管理人員

羅麗紋 博士

電子信箱:llw@gate.sinica.edu.tw

電話:02-7750-5554 或 02-7750-5556

Description

The Nikon Eclipse Ti2-E is an inverted fluorescence microscope system equipped with the high-sensitivity back-illuminated monochrome PCO Edge 4.2 Bi imaging system. This fully motorized XYZ microscope features a multi-channel fluorescence turret , enabling multi-point, multi-fluorescence channel imaging, large-area tiling, and time-lapse photography.

Key Features

  • Rapid large-area image tiling for tissue samples
  • sCMOS camera for capturing low fluorescence signals
  • Multi-channel LED fluorescence light source
  • Suitable for long-term live-cell experiments
  • Various objective lenses available (air, water, and oil immersion)

Specifications

  • Inverted Motorized Microscope: ECLIPSE Ti2-E
  • Motorized Z-axis and XY stage
  • Motorized PFS (Perfect Focus System) for real-time focus maintenance
  • DIC (Differential Interference Contrast) module: 10X and 40X
  • PH (Phase Contrast) module: 10X and 20X
  • Objectives:
    • 10X (DIC)
    • 20X (Ph2)
    • 20X water immersion
    • 40X (DIC)
    • 60X oil immersion
    • 100X oil immersion
  • Fluorescence Filters: DAPI, FITC, Cy5
  • LED multi-band fluorescence light source: switchable between 395/25, 440/20, 470/24, 510/25, 550/15, 575/25, 640/30 nm
  • Imaging System: pco.edge 4.2 bi high-sensitivity back-illuminated monochrome sCMOS camera
  • Image Acquisition and Analysis Software: Nikon NIS-Elements AR

Precautions

  • For slide-mounted samples, use No. 1.5 (0.16-0.19mm) or No. 1.5H (0.17mm) coverslips.
  • Ensure the mounting medium is cured or the coverslip is secured to prevent leakage before imaging.
  • Clean water and oil immersion lenses thoroughly after use.

Assistance Required

Please notify the administrator for assistance with the following:

  1. Non-standard slide-type samples
  2. Use of the live cell incubation system

Location

National Biotechnology Research Park, Building A, 4th Floor, Room A419

Contact Information

Dr. Li-Wen Luo

Email: llw@gate.sinica.edu.tw

Phone: +886-2-7750-5554 or +886-2-7750-5556