自動化細胞活性分析平台
Automatic Living Cell Assay Core Facility
A4-多維度顯微影像分析系統
MD Image Xpress
MD Image Xpress XLS
儀器描述
MD Image Xpress Micro XLS分析系統具有高涵量，即多孔盤式培養之細胞觀察能量。可自動化的於短時間之內對於大量樣品進行螢光的觀察與分析特定細胞族群及其他多項參數。其應用例如細胞凋亡及毒性分析等高涵量分析。
主要特點
- 高通量細胞影像擷取分析
- 6-1536微孔盤或玻片影像
- 經固定處理細胞影像
- 3D影像擷取
- 3D影像量化分析
- Z軸影像掃描
儀器規格
- 支援盤型：6-1536 well多孔盤
- 光源：二階能量式LED燈 (SOLA II light engine): 380-680 nm
- 螢光濾片：DAPI, FITC/Alexa 488, Texas Red, Rhodamine/TRITC, Cy5
- 物鏡：4x, 10x, 20x, 40x
- 相機：Andor Zyla 5.5
- 控制軟體：Meta Xpress Software
- 離線分析軟體：Meta Xpress Software
使用注意事項
- 10x以上鏡頭應用，需使用薄底或玻璃底培養盤
- 以下功能應用不提供自行操作：非一般常用多孔盤型式之樣品
放置地點
國家生技研究園區A棟5樓A512a室
管理人員
陸韻翔 專員
電子信箱：lululoh@gate.sinica.edu.tw
電話：02-7750-5556
Description
MD Image Xpress Micro XLS analysis system is the high-content microplate imaging system. The system could automate the observation and analysis of a large number of samples in a short time. The application could be used to analyze cell apoptosis or dose-dependence experiments.
Application
- High-content cell imaging and analysis
- 6-1536 microplate imaging
- Cell imaging after fixation
- Acquisition of 3D imaging
- Analysis of 3D imaging
- Z-axis imaging scanning
Specification
- Supporting microplate type：6-1536 well
- Light source：Lumencor SOLA II light engine: 380-680 nm
- Filter：DAPI, FITC/Alexa 488, Rhodamine/TRITC, Texas Red, Cy5
- Objective：4x, 10x, 20x, 40x
- Camera：Andor Zyla 5.5, 2048x2048 pixels
- Control software：Meta Xpress Software
- OFF-Line analysis software：Meta Xpress Software
Precautions for user
- Application of 10x or other high-magnification objectives will be suggest to use the thin bottom layer or glass bottom microplate.
- Some functional application is only technician operation：
■ 1. Non-common used microplate
Facility location
National Biotechnology Research Park Building A, 5th Floor, room A512a
Manager
陸韻翔 技術專員
Email：lululoh@gate.sinica.edu.tw
TEL：02-7750-5556