自動化細胞活性分析平台

Automatic Living Cell Assay Core Facility

A3-全時細胞活性分析系統 (Biospa & EL406)

BioTek Cytation5 plus BioSpa8 & EL406

BioTek Cytation5 plus BioSpa8 & EL406

全時細胞活性分析系統 (Biospa & EL406)

儀器描述

Cytation™ 5 結合了自動化數位顯微鏡和微量盤檢測儀。主要支援自動化活細胞培養與時序(time-lapse)造影。
顯微鏡模組可拍攝螢光、明視野、彩色明視野及相位差影像。多功能微量盤檢測儀包含全波長式及濾鏡式的檢測儀,可進行螢光、冷光及可見光偵測。 Gen5™ 軟體可控制自動化影像系統及微量盤檢測儀,並可進行影像及數據分析。

主要特點

  • 6-384 well多孔盤吸光數據讀取
  • 6-384 well多孔盤影像擷取
  • 時間序細胞培養影像擷取
  • CO2/O2 氣體控制、溫控和震盪可應用於活細胞實驗
  • 自動化細胞培養與分注實驗影像擷取

儀器規格

  • 支援盤型:6-384 well
  • 盤式數據偵測 -UV/VIS、螢光、冷光、時差螢光
  • 顯微鏡模組:四色螢光、明視野、彩色明視野、明視野像位差
  • 光源:LED Cube
  • 螢光濾片:DAPI, GFP, RFP, Cy5
  • 物鏡:4x (phase), 10x, 10x (phase) 20x (phase), 40x,及40x (phase)
  • 相機:16-bit Sony CMOS 1.4 Megapixel
  • CO2/O2 氣體控制、溫度和震盪控制
  • 自動化細胞培養及生化反應培養箱:BioSpa8
  • 細胞液更換系統:EL406
  • 控制軟體:BioTek Gen5
  • 離線分析軟體:BioTek Gen5

使用注意事項

  • 以下功能應用不提供自行操作:
     使用BioSpa8自動培養設備 或EL406分注設備
  • 使用活細胞系統另收取氣體費用

放置地點

國家生技研究園區A棟5樓A512室

管理人員

陸韻翔 專員

電子信箱:lululoh@gate.sinica.edu.tw

電話:02-7750-5556

Description

Cytation™ 5 combine the microplate reader and imager, and support the automatic incubation and washer/dispenser system. This system mainly support automatic cell culture combine time-lapse imaging. The microscopic module support fluorescence, brightfield, color brightfield, and phase contrast and microplate reader could support UV/VIS, Fluorescence, Luminescence, Time-resolved fluorescence detection. Finally, Software Gen5™ could control the “Cytation™ 5” and support the imaging analysis.

Application

  • 6-384 well microplate reader
  • 6-384 well microplate imager
  • Long-term Time-lapse cell culture and imaging
  • Controller of the concentration of O2 and CO2, temperature and vibration for living cell experiments
  • Combine automatic cell culture and dispenser system

Specification

  • Supporting microplate type:6-384 well
  • Microplate detection:UV/VIS, Fluorescence, Luminescence, Time-resolved fluorescence
  • Imaging module:Fluorescence, brightfield, color brightfield and phase contrast
  • Light source:LED Cube
  • Filter:DAPI, GFP, RFP, Cy5
  • Objective:4x, 10x, 10x (phase) 20x, 40x, and 40x (phase)
  • Camera:16-bit Sony CMOS, standard or wide FOV
  • Controller for CO2/O2, temperature and vibration
  • Automatic incubator:BioSpa8
  • Washer/Dispenser:EL406
  • Control software:BioTek Gen5
  • OFF-Line analysis software:BioTek Gen5

Precautions for user

  • Some functional application is only technician operation:
    1. Non-common used microplate
    2. Using BioSpa8 incubator or EL406 dispenser

Facility location

National Biotechnology Research Park Building A, 5th Floor, room A512

Manager

陸韻翔 技術專員

Email:lululoh@gate.sinica.edu.tw

TEL:02-7750-5556