自動化細胞活性分析平台
Automatic Living Cell Assay Core Facility
A3-全時細胞活性分析系統 (Biospa & EL406)
BioTek Cytation5 plus BioSpa8 & EL406
BioTek Cytation5 plus BioSpa8 & EL406
儀器描述
Cytation™ 5 結合了自動化數位顯微鏡和微量盤檢測儀。主要支援自動化活細胞培養與時序(time-lapse)造影。
顯微鏡模組可拍攝螢光、明視野、彩色明視野及相位差影像。多功能微量盤檢測儀包含全波長式及濾鏡式的檢測儀,可進行螢光、冷光及可見光偵測。 Gen5™ 軟體可控制自動化影像系統及微量盤檢測儀,並可進行影像及數據分析。
主要特點
- 6-384 well多孔盤吸光數據讀取
- 6-384 well多孔盤影像擷取
- 時間序細胞培養影像擷取
- CO2/O2 氣體控制、溫控和震盪可應用於活細胞實驗
- 自動化細胞培養與分注實驗影像擷取
儀器規格
- 支援盤型:6-384 well
- 盤式數據偵測 -UV/VIS、螢光、冷光、時差螢光
- 顯微鏡模組:四色螢光、明視野、彩色明視野、明視野像位差
- 光源:LED Cube
- 螢光濾片:DAPI, GFP, RFP, Cy5
- 物鏡:4x (phase), 10x, 10x (phase) 20x (phase), 40x,及40x (phase)
- 相機:16-bit Sony CMOS 1.4 Megapixel
- CO2/O2 氣體控制、溫度和震盪控制
- 自動化細胞培養及生化反應培養箱:BioSpa8
- 細胞液更換系統:EL406
- 控制軟體:BioTek Gen5
- 離線分析軟體:BioTek Gen5
使用注意事項
- 以下功能應用不提供自行操作:
■ 使用BioSpa8自動培養設備 或EL406分注設備 - 使用活細胞系統另收取氣體費用
放置地點
國家生技研究園區A棟5樓A512室
管理人員
陸韻翔 專員
電子信箱:lululoh@gate.sinica.edu.tw
電話:02-7750-5556
Description
Cytation™ 5 combine the microplate reader and imager, and support the automatic incubation and washer/dispenser system. This system mainly support automatic cell culture combine time-lapse imaging. The microscopic module support fluorescence, brightfield, color brightfield, and phase contrast and microplate reader could support UV/VIS, Fluorescence, Luminescence, Time-resolved fluorescence detection. Finally, Software Gen5™ could control the “Cytation™ 5” and support the imaging analysis.
Application
- 6-384 well microplate reader
- 6-384 well microplate imager
- Long-term Time-lapse cell culture and imaging
- Controller of the concentration of O2 and CO2, temperature and vibration for living cell experiments
- Combine automatic cell culture and dispenser system
Specification
- Supporting microplate type:6-384 well
- Microplate detection:UV/VIS, Fluorescence, Luminescence, Time-resolved fluorescence
- Imaging module:Fluorescence, brightfield, color brightfield and phase contrast
- Light source:LED Cube
- Filter:DAPI, GFP, RFP, Cy5
- Objective:4x, 10x, 10x (phase) 20x, 40x, and 40x (phase)
- Camera:16-bit Sony CMOS, standard or wide FOV
- Controller for CO2/O2, temperature and vibration
- Automatic incubator:BioSpa8
- Washer/Dispenser:EL406
- Control software:BioTek Gen5
- OFF-Line analysis software:BioTek Gen5
Precautions for user
- Some functional application is only technician operation:
■ 1. Non-common used microplate
■ 2. Using BioSpa8 incubator or EL406 dispenser
Facility location
National Biotechnology Research Park Building A, 5th Floor, room A512
Manager
陸韻翔 技術專員
Email:lululoh@gate.sinica.edu.tw
TEL:02-7750-5556