自動化細胞活性分析平台

Automatic Living Cell Assay Core Facility

A8-高內涵影像篩選系統

PerkinElmer Opera Phenix High-Content Screening System

Opera Phenix

專人/專案諮詢 網路預約
高內涵影像篩選系統

儀器描述

PerkinElmer Opera Phenix高內涵影像篩選系統可進行高通量的細胞型態篩選，透過隨附軟體的分析功能可進行批次的大量細胞影像型態分析進行藥物篩選。

主要特點

  • 雷射光源與穿透光光源
  • 可進行三螢光通道影像的同步擷取，最大化減少實驗所需時間
  • 高通量細胞影像型態分析
  • 6-1536多孔盤或玻片影像
  • 配備高NA水鏡，可獲得更佳的成像品質
  • Widefield與confocal成像模式
  • 活細胞影像
  • 經固定處理細胞影像
  • 拍攝時可控溫並維持二氧化碳濃度的培養箱
  • 3D影像擷取
  • 3D影像量化分析
  • Z軸影像掃描

儀器規格

  • 支援盤型：6-1536 well多孔盤，透過轉接支架可支援玻片拍攝
  • 光源：固態雷射(solid state lasers):UV: 375 nm, Blue: 488 nm, Green/Yellow: 561 nm, Red: 640 nm；LED光源: 740 nm
  • 螢光濾片：Cam1: 435-480 nm or 435-550 nm, Cam2: 500-550 nm, Cam3: 570-630 nm, Cam4: 650-760 nm
  • 物鏡：1.25xA, 10xA, 20xA, 40xA, 20xW, 40xW
  • 相機：16 bit sCMOS 4.7 Megapixel (2160x2160), 6.5 μm pixel size
  • 控制軟體：Harmony High-Content Imaging and Analysis Software
  • 分析軟體：Harmony High-Content Imaging and Analysis Software

使用注意事項

  • 使用非高內涵影像專用多孔盤，可能會有盤子最外圍的well無法拍攝的情形

  • 多孔盤的高度不可超過2公分

  • 使用活細胞系統另收取氣體費用

此儀器目前暫不提供自行操作

放置地點

A609室

管理人員

陸韻翔 技術專員

Email：lululoh@gate.sinica.edu.tw

TEL：02-7750-5556

Description

The PerkinElmer Opera Phenix high-content screening system is designed to carry out high-throughput cellular phenotype screening.

Application

  • Laser light source and penetrating light source
  • Capture images in threefluorescent channels simultaneously, minimize the time required for experiment
  • High-throughput cell image phenotypic analysis
  • 6-1536 well microplate plate or slide image
  • Equipped with high NA water mirrors for better imaging quality
  • Widefield and confocal imaging modes
  • Live cell imaging
  • Fixed cell imaging
  • An incubator capable of controlling temperature and maintaining carbon dioxide concentration during imaging
  • 3D image capture
  • 3D image quantitative analysis
  • Z-axis image scanning

Specifications

  • Supported plate type: 6-1536 well microplate, supports slide imaging with adapter.
  • Light source: solid state lasers: UV: 375 nm, Blue: 488 nm, Green/Yellow: 561 nm, Red: 640 nm; LED light source: 740 nm.
  • Fluorescent filter: Cam1: 435-480 nm or 435-550 nm. Cam2: 500-550 nm, Cam4: 650-760 nm.
  • Objective lens: 1.25xA, 10xA, 20xA, 40xA, 20xW, 40xW.
  • Camera: 16 bit sCMOS 4.7 Megapixel (2160x2160), 6.5 μm pixel size.
  • Control software: Harmony High-Content Imaging and Analysis Software.
  • Analysis software: Harmony High-Content Imaging and Analysis Software.

Notes for use

  • The outer surrounding well may not available for imaging while using a microplate not designed for high-content screening.
  • The height of microplate cannot exceed 2 cm.

This instrument currently does not provide self-operation.

Facility location

The instrument is temporarily placed in Room 645, 6th Floor, Interdisciplinary Research Building for Science and Technology.

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