細胞分選及分析平台

Cell Sorting and Analysis Core Facility

F13-細胞相位差顯微鏡

Nikon Eclipse Ts2

Nikon Eclipse Ts2

介紹

 

流式細胞技術(flow cytometry)是一種用於懸浮液體中對微小顆粒計數與分選的生物學技術。在液流的狀態下,將通過光學或電子檢測器的一個個細胞進行連續的多種參數分析。利用其物理特性與化學性等包括顆粒大小、顆粒性、或其內部結構以及相對之螢光信號強度來實現高速、逐一的細胞定量分析和分選的技術。此技術可以檢測的項目非常多且持續增加中,如:細胞的體積和形態複雜程度、DNA(細胞周期分析、細胞凋亡、細胞增殖等)、細胞表面抗原(CD標記)、胞內抗原(各種cytokine、次級媒介等)、胞內離子化的鈣、鎂,膜電勢..等。

細胞相位差顯微鏡

 

Nikon 以它領先業界的CFI60無限遠光學系統、LED穿透光及螢光模組來提升這部全新倒立顯微鏡的光學品質。新的ECLIPSE Ts2顯微鏡提供了更出色清晰的觀察影像及人性化的操控規格,實現更有效率的活細胞觀察 。

 

產品規格

  • 目镜(F.O.V.):10x (22), 12.5x (16), 15x (14.5)
  • 調焦:通過物鏡轉換器向上/向下運轉
    上7mm,下1.5mm
    粗:每轉37.7mm, 精行程:每轉0.2mm粗運動力矩可调
  • 物鏡轉盤:五孔物鏡轉換器
  • 聚光鏡:ELWD聚光鏡(NA0.3,W.D.75mm)
  • 物鏡:10X,20X,40X
  • 載物台大小:170 (X) x 247 (Y) mm
  • 尺寸:236 (W) ×548 (D) ×471 (H) mm
  • 重量:13kg
  • 電壓/電流:100V-240VAC±10%、50/60Hz、0.35A
  • 耗電量:15W

放置地點

國家生技研究園區A棟5樓A511室

管理人員

技術專員: 毛普威

電子信箱:pwmao105grc@gate.sinica.edu.tw

電話:02-7750-5500 分機 1509 (A505流式細胞分析室)

               02-7750-5500 分機 1514 (A511 流式細胞分選室)

 

Features

  • Efficient and comfortable observation
    LED light sources and built-in Diascopic/Epi-fluorescence illumination systems
    reduce setup time and allow users to concentrate on their research.
  • Faster, brighter images with LED illumination
    The LED light source is alignment-free, resulting in faster setup and consistent results. LEDs also eliminate frequent bulb replacements, saving the user time and money. Moreover, the new Contrast Shield (optional) provides high signal-to-noise fluorescence observation even in brightly lit culture rooms.
  • Streamlined Operation
    Control buttons on the Ts2 microscope are intuitively located for a streamlined workflow. Commonly used controls such as the on/off and Diascopic/Epi-fluorescence switching buttons are located at the front panel for easy reach. Buttons pertaining to either Diascopic or Epi-fluorescence control are zoned to the left and right sides of the microscope body, respectively, to eliminate confusion and improve workflow efficiency.
  • Easy-to-use Mechanical Stage
    The high performance mechanical stage (optional) can accommodate a wide range of flasks and cell culture chambers. The new stage design also directly accommodates well plates. In addition, the sample holder is easily removed to accommodate large flasks.
  • "Emboss Contrast" Contrasting Technique
    Emboss Contrast is a cost-effective optical technique which does not require costly optics. Utilizing just a bright-field objective lens and two contrast sliders, Emboss Contrast provides pseudo-three dimensional and glare-free images for thick specimens such as iPS cells which would normally suffer from halos with conventional phase contrast methods. Additionally, Emboss Contrast is compatible with both glass and plastic culture chambers, making it a very versatile observation technique.
  • LED-based high-quality Diascopic and Epi-fluorescence observation
    Two models are available to meet your needs; a Diascopic illumination model, the Eclipse Ts2, and an Epi-fluorescence illumination model, the Eclipse Ts2-FL. High-intensity LED sources are employed for both Diascopic and Epi-fluorescence illumination. The built-in fly-eye-lens ensures uniform brightness across the entire field of view.
  • Compact and highly stable body
    Illumination modules including the Epi-fluorescence light source have been seamlessly incorporated into the microscope main body, resulting in a compact and simple design form that's also durable. The compact structure is also vibration-resistant to provide highly stable sample observations.
    The camera port is located on the side of the microscope to provide unimpeded viewing of the stage even when placed inside a culture hood.

Specifications

  • Eyepiece (F.O.V.):10X (22) , 15X (16), 20X (12.5)
  • Focusing:Via nosepiece up/down movement, Stroke (manual): Up 7 mm down 1.5 mm
    Coarse stroke: 37.7 mm per rotation, Fine stroke: 0.2 mm per rotation, Coarse motion torque adjustable
  • Nosepiece:Quintuple nosepiece
  • Condenser:ELWD Condenser (NA 0.3、W.D. 75 mm)
  • Slider:
    Precentered or Centering PH Slider, 10X, 20X, 40X objectives available for Phase Contrast
    Emboss Contrast sliders (both condenser-side slider and eyepiece-tube-side slider must be mounted), 10X, 20X, 40X objectives available for Emboss Contrast
  • Stage
    Plain Stage, stage size: 170 (X) x 247 (Y) mm
    With Acrylic Type of Stage Ring
    Mechanical stage (optional), stroke:126 (X) x78 (Y) mm
    Accepts 5 types of micro-testplate, well clamper and stage clip
  • Dimensions:236 (W) ×548 (D) ×471 (H) mm
  • Weight (approx.) :13 kg
  • Rated Voltage/Electric Current:100V-240VAC±10%、50/60Hz、0.35A
  • Power Consumption:15W

Site

National Biotechnology Research Park Building A, 5th Floor, room A511